Following the Forests: How the Variable Antshrike spread around the Amazon

Genetic analyses help to explain the peculiar circum-Amazonian distribution of this species.

In February 2020, I started a new position as lecturer at Wageningen University (the Netherlands). I was looking forward to interacting with students during a variety of ecology courses. Then the Corona-virus spread across Europe and I was forced to start teaching online. Although the switch to an online format was challenging, I tend to focus the bright side and reflect on all the new skills I learned during the past months. For example, I recently recorded two knowledge clips about the evolution of birds that you can watch on YouTube (on the origins of feathered flight and the diversity in beak morphology).

Today, I came across a TEDxtalk by Aaron Barth about the importance of story-telling in online education. He criticized the one-way style of teaching where an instructor gives a bullet-point-rich lecture and provides some reading material. Teaching (and by extension, science communication in general) should be interactive and can benefit from storytelling. That is also something I try to do on this blog – for instance, I announce new blog posts as “Avian Hybrids stories”. In this post, I will share the evolutionary story of the Variable Antshrike (Thamnophilus caerulescens), based on a recent study in the journal Molecular Phylogenetics and Evolution.

The Variable Antshrike © Dario Sanches | Wikimedia Commons

 

Around the Amazon

As the name suggests, the Variable Antshrike shows extensive plumage variation. Another interesting feature about this species is its peculiar distribution. Populations are namely wrapped around the Amazon – spanning the Atlantic Forest, Cerrado, Chaco, and the foothills of the Andes – culminating in a so-called circum-Amazonian distribution (see map below). This geographic arrangement does not fit neatly into the classical South American domains, such as Amazonia, the Atlantic Forest or the Llanos.

What processes led to this circum-Amazonian distribution? The evolutionary history of South American birds is thought to be shaped by two main processes: geological and climatic events that affected the spread of tropical forests and the birds living in them (the Forest Refugia Hypothesis) or the isolating effects of rivers (the Riverine Barrier Hypothesis). Do these hypotheses also apply to the Variable Antshrike?

The circum-Amazonian distribution of the Variable Antshrike. From: Bolívar-Leguizamón et al. (2020) Molecular Phylogenetics and Evolution

 

Three Groups

Sergio Bolívar-Leguizamón and his colleagues extracted DNA from 53 museum specimens, representing all known subspecies of the Variable Antshrike. Genetic analyses of one mitochondrial gene and the flanking regions of several ultraconserved elements (UCEs) uncovered three main geographical groups. If you are not familiar with the different South American regions, you can compare the colors on the phylogenetic tree below with the distribution map of the Variable Antshrike. But I will walk you through this circum-Amazonian trip.

The caerensis group contains individuals from the Atlantic Forest north of the river Rio São Francisco (in darkblue). The caerulescens group is composed of samples from southeastern Cerrado and central Atlantic Forest (in orange, green, and purple). Finally, the aspersiventer group spans the transition from the drier environments in the Chaco and southern Yungas in Argentina, Bolivia, and Paraguay (in red and grey) to the more humid forests in the northern Yungas and Central Andes in northern Bolivia and Peru (in pink and lightblue).

The phylogenetic tree of the Variable Antshrike, based on mtDNA. The colors correspond to different subspecies. From: Bolívar-Leguizamón et al. (2020) Molecular Phylogenetics and Evolution

 

Pleistocene Climate

To reconstruct the evolutionary history of these three main genetic clusters, the researchers ran several demographic models. The results from these analyses provided them with divergence times, patterns of gene flow and past population dynamics. It seems that the evolution of this passerine was mainly driven by the expansion and contraction of forest habitat, supporting the Forest Refugia Hypothesis. Putting all this information together, we can tell the story of the Variable Antshrike.

The results of our analyses suggest that the history of T. caerulescens began in the Late Miocene-Pliocene, with an initial widespread population distributed across the Cerrado, Atlantic Forests, the Chaco and the central Andes. During the Early – Middle Pleistocene (0.81–0.59 Ma), climatic fluctuations promoted expansions and contractions of forested habitats separating populations in the northern Atlantic Forest from those farther south. During the Middle Pleistocene (0.50–0.36 Ma), the continued effects of wet-dry cycles caused the contraction of populations in the southern Atlantic Forest and in the Chaco-Andes, but at the same time allowed sufficient opportunities to maintain gene flow via the onset of dry-forest corridors that connected these two regions.

Sounds like a wonderful, ornithological bedtime story.

 

References

Bolívar-Leguizamón, S. D., Silveira, L. F., Derryberry, E. P., Brumfield, R. T., & Bravo, G. A. (2020). Phylogeography of the Variable Antshrike (Thamnophilus caerulescens), a South American passerine distributed along multiple environmental gradients. Molecular Phylogenetics and Evolution148, 106810.

Feature image © Francesco Veronesi | Wikimedia Commons

 

This paper has been added to the Thamnophilidae page.

Figuring out the origin of two Fire-eye Antbird species in the Atlantic Forest

Genetic study uncovers a complicated series of events.

A common mistake in discussions is the “False Dilemma”, which states that if X is wrong than Y must be true. Creationists often apply this style of reasoning when attacking evolutionary theory. They claim that if evolution is wrong, then creationism must be true (which explains why they put so much effort in discrediting evolution). This statement is obviously not true: an inconsistency in the current evolutionary theory does not automatically support creationism.

But let’s not get caught up in this useless and silly debate, because there is a much more interesting discussion about the origin of bird species in the Atlantic Forest of South America. Two main hypotheses have been put forward to explain how new species arise in this region. The refuge hypothesis states that during the Pleistocene vast stretches humid forests were replaced with dry vegetation, creating isolated forest patches in which species diversified. The river-barrier hypothesis, however, focuses on the role of rivers as isolating barriers between populations. It seems that there are only two possibilities: refuges versus rivers. But a recent study in the journal Molecular Phylogenetics and Evolution revealed that this is a false dilemma. There is another option to consider.

A White-shouldered Fire-eye in Brazil © Dario Sanches | Wikimedia Commons

 

Refuges and Rivers

Using genetic data, Manuelita Sotelo-Muñoz and her colleagues reconstructed the evolutionary history of two Atlantic Forest species: the white-shouldered fire-eye (Pyriglena leucoptera) and the fringe-backed fire-eye (P. atra). The analyses revealed that these species diverged about 260,000 years ago, probably driven by habitat fragmentation. The species recently established secondary contact, resulting in the exchange of genetic material. Clearly, these fire-eyes were not isolated long enough for reproductive isolation to evolve. All in all, this scenario seems to support the refuge hypothesis. But wait, there is more…

Within the white-shouldered fire-eye, the researchers uncovered more fine-grained population structure. This species can be divided into three genetically distinct populations: a northern, central and southern lineage. Some of these populations come into contact around the major rivers in the Atlantic Forest. The northern and central population meet at the interfluvium of the de Contas and Pardo rivers, while the southern and the central population mix at the Doce and Grande rivers. These results support the river hypothesis, right? Well, not quite…

The distribution and genetic structure of the white-shouldered and the fringe-backed fire-eye in the Atlantic Forest. Notice the hybrid zones between the different populations (locations 25-27 and 37-44). From Sotelo-Muñoz et al. (2020) Molecular Phylogenetics and Evolution

 

Extinction and Dispersal

A detailed look at the genetic patterns points to a complicated scenario. After divergence from the fringe-backed fire-eye, individuals from the white-shouldered fire-eye spread southwards and diversified into several populations. At some point, the population north of the Pardo river went extinct. This gap was later filled when birds from the central population dispersed northwards. Similarly, the southern populations originated when birds from the central population dispersed southwards. Later on, these populations re-established contact at the rivers. These rivers probably limited dispersal, accentuating the genetic differences between the populations.

Based on this information, the authors argue that “our results support neither the river-barrier nor the refuge hypothesis as originally conceived. Here, dispersal as opposed to vicariance, seems to be the main cause of intraspecific differentiation.” As I mentioned in the beginning of this blog post, there is more to evolution in the Atlantic Forest than refuges and rivers. Don’t ignore dispersal.

 

References

Sotelo-Muñoz, M., Maldonado-Coelho, M., Svensson-Coelho, M., dos Santos, S. S., & Miyaki, C. Y. (2020). Vicariance, dispersal, extinction and hybridization underlie the evolutionary history of Atlantic forest fire-eye antbirds (Aves: Thamnophilidae). Molecular Phylogenetics and Evolution, 106820.

 

This paper has been added to the Thamnophilidae page.