Genetic evidence for hybridization between Magellanic and Humboldt penguins

Several genetic markers are useful to identify hybrids and backcrosses.

A few months ago, I published a scoring scheme to assess the reliability of hybrid reports (see this blog post). In short, this scheme is based on three criteria: (1) the observation of a putative hybrid with photographic evidence or a detailed description, (2) thorough morphological analyses in which the putative hybrid is compared with potential parental species, and (3) genetic analyses of the putative hybrid with reference material from potential parental species. To express the varying levels of confidence that each of these criteria provide, I weighted them differently in the final score for a putative hybrid, namely one point for an observation, two for a morphological analysis, and three for a genetic test. The final tally of these three criteria (ranging from 0 to 6 points) will indicate the level of confidence for a particular hybrid combination. I applied this scheme to the tinamous (family Tinamidae), resulting in one well-documented case and three doubtful records that require further investigation.

The goal is to apply this approach to other bird families in order to provide a better overview of the incidence and reliability of bird hybrids. A recent study in the journal Genetica summarized the literature on penguin hybrids and indicated that most hybridization studies were “based solely on morphological or nesting observations, with no genetic confirmation of hybridization.” In the context of the scoring scheme, most cases of penguin hybrids would thus receive a reliability score between 0 and 3 points. Clearly, more genetic studies are needed to determine the incidence of penguin hybrids. And the study in Genetica delivers a nice example for hybrids between Magellanic Penguins (Spheniscus magellanicus) and Humboldt Penguins (Spheniscus humboldti).

Genetic Markers

Eric Hibbets, Katelyn Schumacher and their colleagues focused on six individuals that were sampled at three colonies from the Atlantic Ocean basin (Caleta Valdés, Punta Tombo, and Cabo Dos Bahías). These birds were initially noted down as Magellanic Penguins, but were later identified as putative hybrids based on the presence of mitochondrial variants (of the COI gene) that are characteristic for Humboldt Penguins. The researchers tested this conclusion with three additional genetic markers: a set of six microsatellites, the immune gene DRβ1 and the sex-linked gene CDH1. They sequenced these markers for several reference samples from both species. The analyses revealed that “three of the four markers (COI, microsatellites, and DRß1) were informative because they provided both Magellanic and Humboldt species-specific alleles or haplotypes that could be used to trace species ancestry in hybrid individuals.”

Distribution ranges of Magellanic (S. magellanicus; light gray stripes) and Humboldt (S. humboldti; dark gray) penguins during the breeding season. Reference populations of Magellanic penguins include samples from three colonies in the Atlantic Ocean (Caleta Valdés, Punta Tombo, and Cabo Dos Bahías). From: Hibbets et al. (2020) Genetica

Backcrosses

What about the six putative hybrids? It turned out that four individuals were backcrosses with some degree of genetic introgression from Humboldt Penguins. The remaining two individuals were actually Humboldt penguins instead of hybrids. These results highlight the value of genetic analyses in hybrid detection. Morphological characters or field observations are not always reliable.

Detailed analyses revealed more admixed individuals among the reference samples. Five out of 37 penguins showed some genetic signs of past hybridization events. Interestingly, all five samples come from the Puñihuil colony, which holds a significant number of intermixed nesting sites of Magellanic and Humboldt penguins. More expeditions will probably uncover more penguin hybrids. And not just between Magellanic and Humboldt Penguins. A recent genomic study reported gene flow between several other species (see this blog post for the details). Who know what penguin hybrids will be discovered with genetic data.

Structure analysis of microsatellite and MHC loci of Magellanic and Humboldt penguin samples. Vertical bars represent individuals with assignment probabilities (Q, y-axis) to the Magellanic (gray) and Humboldt (white) populations. The grey and white horizontal bars below the assignment probabilities represent corresponding species-specific mitochondrial haplotypes of those individuals as determined by COI sequences. Asterisks (*) indicate individuals of hybrid origin that were identified by this study. From: Hibbets et al. (2020) Genetica

References

Hibbets, E. M., Schumacher, K. I., Scheppler, H. B., Boersma, P. D., & Bouzat, J. L. (2020). Genetic evidence of hybridization between Magellanic (Sphensicus magellanicus) and Humboldt (Spheniscus humboldti) penguins in the wild. Genetica148(5), 215-228.

Featured image: Magellanic penguin (Spheniscus magellanicus) © David | Wikimedia Commons

This paper has been added to the Sphenisciformes page.

Where did all these penguins come from?

Genomic analyses unravel the evolutionary history of these flightless diving birds.

The evolution of penguins (order Sphenisciformes) remains a mystery. Different genetic studies disagree about the evolutionary relationships between particular species, the timing of speciation events and the original distribution of these iconic seabirds. The divergence time of the crown group (all living representatives of the penguins) ranges from 9.9 million years ago (during the Miocene) to 47.6 million years ago (during the Eocene). And the exact area of origin is also a matter of debate: some ornithologists suggest Antarctica with a subsequent expansion into warmer waters, while others point to Australia or New Zealand followed by colonization of the colder Antarctica. One way to settle these debates is to bring out the big guns: genomic data. A recent study in the journal PNAS applied this strategy and analyzed 22 genomes, representing 18 penguin species. Time to find out what they discovered about the evolution of penguins – a word that Benedict Cumberbatch has some difficulty pronouncing (see video below).

From Australia to Antarctica?

Let’s start with the most likely area of origin for penguins. The authors reconstructed the ancestral distributions of the sampled species and identified the coastlines of Australia, New Zealand, and nearby islands as the original range of the ancestor of extant penguins. From there, these birds colonized Antarctica and South America where they diversified into several species. The genomic analyses provide estimates for the timing of these events.

The first branching event led to the establishment of the genus Aptenodytes in the Antarctic, and reconstructions of the ancestral Pygoscelis species indicate that they colonized the Antarctic Peninsula soon after Aptenodytes, pointing to a long history of Antarctic occupation. In the mid-Miocene, the lineage leading to the Spheniscus/Eudyptula ancestor colonized the South American coast, with members of the genera Eudyptes, Eudyptula, Megadyptes, and Spheniscus progressively diversifying and colonizing warmer at-sea environments.

During the cooling event at the transition of the Pliocene and Pleistocene (about 2.5 million years ago), ice shelves expanded across the Southern Ocean, probably reducing connectivity between several penguin populations. This culminated in more speciation events within the genera Pygoscelis, Spheniscus, Eudyptes and Aptenodytes. The figure below provides a nice overview of all these events.

The evolutionary history of penguins based on genomic data. Reconstruction of ancestral distributions (the colored letters on the nodes in the tree) suggest that the ancestor of modern penguins lived in Australia and New Zealand. From: Vianna et al. (2020) PNAS.

Genes Going with the Flow

The phylogenetic tree from the genomic analyses largely agreed with another recent study based on complete mitochondrial genomes (which I also covered on this blog). A few differences between these studies can be explained by hybridization between several penguin species. The researchers write that “some of the main episodes of genomic introgression were detected among erect-crested and the ancestral rockhopper penguin species (17 to 23%), erect-crested and macaroni/royal penguins (25%), and the Galápagos/Humboldt ancestor and Magellanic penguins (11%).” Interestingly, the direction of introgression in some of these species followed the clockwise flow of the Antarctic Circumpolar Current, the ocean current that circles around Antarctica. Individual penguins might have drifted off during foraging trips and were transported to nearby populations where they interbred with another resident species. Quite literally, gene flow.

This study nicely illustrates the power of genomic analyses. There is an enormous treasure of information hidden in the seemingly meaningless strings of A, T, G and C. With clever methods and careful analyses we are now able to find meaning in these DNA sequences and reconstruct the wonderful evolutionary history of life on our planet.

Patterns of introgression between different penguins species and their ancestors. From: Vianna et al. (2020) PNAS.

References

Vianna, J. et al. (2020). Genome-wide analyses reveal drivers of penguin diversification. Proceedings of the National Academy of Sciences117(36), 22303-22310.

Featured image: King Penguins (Aptenodytes patagonicus) © Ben Tubby | Wikimedia Commons

This paper has been added to the Sphenisciformes page.